Abstracts
- 09/01/2012 - V. A. Huynh-Thu
- 09/01/2012 - R. Küffner
- 09/01/2012 - C. Ambroise
- 16/09/2011 - M. Bunster
- 27/04/2011 - P. Meyer
- 06/04/2011 - D. Débois
- 30/03/2011 - J. Wouters
- 28/01/2011 - J.-C. Lambert
- 13/12/2010 - J. Giard
- 17/11/2010 - B. Charloteaux
- 12/05/2010 - P. Soumillion
- 30/04/2010 - J.-P. Vert
- 14/04/2010 - X. Tordoir
- 24/03/2010 - J.C. Voegel
- 17/03/2010 - A. Turtoi
- 24/02/2010 - L. Palmeira
- 27/01/2010 - D. Devos
- 09/12/2009 - F. Heuze
- 25/11/2009 - L. Geris
- 19/11/2009 - V. Gabelica
- 14/10/2009 - G. Zocchi
- 30/09/2009 - K. Van Steen
- 12/06/2009 - D. Gianola
- 05/06/2009 - F. J. Theis
- 02/06/2009 - M. Babu
- 07/05/2009 - J. Eyzaguirre
- 22/04/2009 - E. Bullinger
- 08/04/2009 - F. Francis
- 04/03/2009 - Kick-off
Application of proteomic tools to investigate symbiotic associations in insects
Frederic Francis
Date and place: Wednesday April, 8th 12:30 pm at Amphi Sternon (CHU, B36)
Proteomics found recent developments due to significant improvement and availability of both protein separation and identification methods. Moreover, functional information’s linked to the studied proteins was brought when compared to genomic approach. For these reasons, molecular entomologists have now a panel of tools to determine the proteome patterns related to differential adaptation of insects to cope with their environment such as plant defence mechanisms. Two models will be used as case studies : (1) the aphid and related symbiotic bacteria in relation to host plant adaptation and (2) the termites and the diversified symbionts (protists, bacteria, yeast) in regard to ligno-cellulosic digestion ability.
The adaptation and metabolic changes of insects , aphids or termites, in relation to host plants or feeding materials focusing on the role of the symbionts was investigated. Use of artificial diet including diverse antibiotics but also the comparison of proteomes related to whole insects and respective purified symbionts were studied to identify the respective origin and function of proteins constituting the studied proteomes. Diverse methods including traditional two dimension electrophoresis, 2D-Differencial In Gel Expression (2D-Dige), liquid chromatography (LC) coupled with mass spectrometry (ESI-MS-MS and Maldi-Tof-MS-MS) and data bank investigations were developed.
From the proteome investigation and identification from aphid fed with particular antibiotics but also from proteomes of whole insects and related extracted symbionts, particular proteins of interest were selected and accurately characterised with both fundamental but also applied views. This broad proteomic approach will be discussed as an interesting and reliable tool to study the biologically involved proteins from insects in response to several environmental changes, and particularly the insect - host plant interactions.